Product Name :BID (phospho-S78) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :IHC: 1:50~1:200
Background :BID, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-BID (S78) polyclonal antibody detects endogenous levels of BID protein only when phosphorylated at Ser78.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,Death Receptor Signaling,
Alternative Name :BH3-interacting domain death agonist; p22 BID; BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID;,BH3-interacting domain death agonist p11; p11 BID; BID
Immunogen :Synthetic phosphopeptide derived from human BID around the phosphorylation site of Serine 78.
Modification :Phosphorylation
