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BRCA1 (phospho-S1524) polyclonal antibody

货号: BS4027

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4027

Product Name :BRCA1 (phospho-S1524) polyclonal antibody

Swiss-Prot :P38398

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-BRCA1 (S1524) polyclonal antibody detects endogenous levels of BRCA1 protein only when phosphorylated at Ser1524.

BiowMW :~ 200 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :Breast cancer type 1 susceptibility protein; RING finger protein 53; BRCA1; RNF53

Immunogen :Synthetic phosphopeptide derived from human BRCA1 around the phosphorylation site of Serine 1524.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of BRCA1 (phospho-S1524) polyclonal antibody at 1:500 dilution Lane1:A2780 whole cell lysate(40ug) Lane2:SK-OVCAR3 whole cell lysate(40ug) Lane3:The Lung tissue lysate of Mouse(40ug) Lane4:H1792 whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug)

Autophagy-deficiency in hepatic progenitor cells leads to the defects of stemness and enhances susceptibility to neoplastic transformation

JournalCancer Letters

ApplicationWB

IF6.508

SpeciesRat

PMID26607902