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Bcl-X (phospho-S62) polyclonal antibody（KU）

货号: BS4025

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4025

Product Name :Bcl-X (phospho-S62) polyclonal antibody（KU）

Swiss-Prot :Q07817

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:150

Background :Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Bcl-x, one of several additional proteins with sequence homology to Bcl-2, is expressed as Bcl-xL, a 233 amino acid protein with 43% sequence identity with Bcl-2 that suppresses cell death, and Bcl-xS, a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188) found in Bcl-xL and which functions as a dominant inhibitor of Bcl-2. A further apoptosis-inducing protein, Bad, dimerizes both with Bcl-xL and to a lesser extent with Bcl-2, thus displacing Bax and inducing apoptosis

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Bcl-X (S62) polyclonal antibody detects endogenous levels of Bcl-X protein only when phosphorylated at Ser62.

BiowMW :~ 26 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1; BCL2L; BCLX; Bcl-xL; Bcl-XS; BclxL; BclXS

Immunogen :Synthetic phosphopeptide derived from human Bcl-X around the phosphorylation site of Serine 62.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of Bcl-X (phospho-S62) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:The Brain tissue lysate of Mouse(40ug) Lane4:The Brain tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of p-Bcl-X (S62) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells

JournalOncogene

ApplicationWB

IF6.634

SpeciesHuman

PMID29520102
Methylcrotonyl-CoA carboxylase subunit 1 (MCCA) regulates multidrug resistance in multiple myeloma

JournalLIFE SCIENCES

ApplicationWB

IF6.1

SpeciesHuman

PMID37805164