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Bcl-2 (phospho-S70) polyclonal antibody（KU）

货号: BS4023

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4023

Product Name :Bcl-2 (phospho-S70) polyclonal antibody（KU）

Swiss-Prot :P10415

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kDa, membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Bcl-2 (S70) polyclonal antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at Ser70.

BiowMW :~ 33 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,Death Receptor Signaling,

Alternative Name :Apoptosis regulator Bcl-2; Bcl 2; BCL2

Immunogen :Synthetic phosphopeptide derived from human Bcl-2 around the phosphorylation site of Serine 70.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of Bcl-2 (phospho-S70) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 1h whole cell lysate(40ug) Lane3:HEK293T treated with UV for 6h whole cell lysate(40ug) Lane4:HEK293T treated with UV for 24h whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Bcl2 (S70) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

MiR-449a regulates autophagy to inhibit silica-induced pulmonary fibrosis through targeting Bcl2

JournalJournal Of Molecular Medicine-Jmm

ApplicationWB

IF4.427

SpeciesMouse,Human

PMID27351886
MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesRat

PMID26252661