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Bad (phospho-S136) polyclonal antibody（KU）

货号: BS4021

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4021

Product Name :Bad (phospho-S136) polyclonal antibody（KU）

Swiss-Prot :Q92934

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Bad (S136) polyclonal antibody detects endogenous levels of Bad protein only when phosphorylated at Ser136

BiowMW :~ 24 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :BAD, Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8

Immunogen :Synthetic phosphopeptide derived from human Bad around the phosphorylation site of Serine 136.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Bad (phospho-S136) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 1h whole cell lysate(40ug) Lane3:HEK293T treated with UV for 4h whole cell lysate(40ug) Lane4:HEK293T treated with UV for 16h whole cell lysate(40ug) Lane5:HEK293T treated with UV for 24h whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Bad (S136) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

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