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ASCL1 (E158) polyclonal antibody（KU）

货号: BS3688

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS3688

Product Name :ASCL1 (E158) polyclonal antibody（KU）

Swiss-Prot :P50553

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:1000~1:2000

Background :Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ASCL1 (E158) polyclonal antibody detects endogenous levels of ASCL1 protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Achaete-scute homolog 1; ASH-1; Hash1; Class A basic helix-loop-helix protein 46; bHLHa46

Immunogen :Synthetic peptide, corresponding to Human ASCL1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ASCL1 (E158) polyclonal antibody at 1:1000 dilution Lane1:The brain tissue lysate of mouse(30ug) Lane2:The brain tissue lysate of Rat(30ug) Lane3:K562 whole cell lysate(30ug) Lane4:BV2 whole cell lysate(30ug) Lane5:A2780 cell membrane lysate(30ug)

Abnormal cerebellar development and Purkinje cell defects in Lgl1-Pax2 conditional knockout mice

JournalDevelopmental Biology

ApplicationWB

IF2.936

SpeciesMouse

PMID25050931