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PI3K p85α (Q498) polyclonal antibody（KU）

货号: BS3678

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS3678

Product Name :PI3K p85α (Q498) polyclonal antibody（KU）

Swiss-Prot :P27986

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PI 3-kinase p85α (Q498) polyclonal antibody detects endogenous levels of PI 3-kinase p85α protein.

BiowMW :~ 85 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1; GRB1

Immunogen :Synthetic peptide, corresponding to amino acids 470-510 of Human PI3K p85α.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PI3K p85α (Q498) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:The Brain tissue lysate of Rat(40ug) Lane5:The Lung tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of PI 3-kinase p85α (Q498) pAb in paraffin-embedded human lung carcinoma tissue.

Immunofluorescence analysis of MCF-7 cells using PI 3-kinase p85α (Q498) pAb at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using KPNA1 antibody at dilution of 1:100 (40x lens).

Sericin inhibits MDA‑MB‑468 cell proliferation via the PI3K/Akt pathway in triple‑negative breast cancer

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesHuman

PMID33313947
Neuregulin-1β attenuates sepsis-induced diaphragm atrophy by activating the PI3K/Akt signaling pathway

JournalJournal Of Muscle Research And Cell Motility

ApplicationWB

IF1.737

SpeciesRat

PMID30989579
Unveiling the mechanisms of mechanical loading-induced knee osteoarthritis through transcriptomics

JournalINTERNATIONAL IMMUNOPHARMACOLOGY

ApplicationWB

IF4.8

SpeciesRat

PMID40327988