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ERK1/2 (H197) polyclonal antibody

货号: BS3628

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS3628

Product Name :ERK1/2 (H197) polyclonal antibody

Swiss-Prot :P27361/P28482

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB 1:500 - 1:1000 IHC 1:50 - 1:100 IP 1:10 - 1:100

Background :The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ERK1/2 (H197) polyclonal antibody detects endogenous levels of ERK1/2 protein.

BiowMW :~ 42,44 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1; ERK2; PRKM1; PRKM2

Immunogen :Synthetic peptide, corresponding to amino acids 165-210 of Human ERK1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ERK1/2 (H197) at 1:2000 dillution Lane1:Hela whole cell lysate(15μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(15μg)

Immunohistochemistry of paraffin-embedded mouse heart using TMEM173 antibody at dilution of 1:100 (x40 lens).

Immunoprecipitation of the Brain tissue lysate of Rat using ERK1/2 (H197) pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using ERK1/2 (H197) pAb #BS3628.“↑”(supernatant); “↓”(deposition)

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