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mTOR (S2442) polyclonal antibody（KU）

货号: BS3611

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS3611

Product Name :mTOR (S2442) polyclonal antibody（KU）

Swiss-Prot :P42345

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP IF

Application_all :WB:1:500~1:1000 IF: 1:50~1:200 IP:1:50~1:200

Background :mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :mTOR (S2442) polyclonal antibody detects endogenous levels of mTOR protein.

BiowMW :~ 289 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Immunogen :Synthetic peptide, corresponding to Human mTOR.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of mTOR (S2442) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)

Immunoprecipitation of HEK293T cell lysates using mTOR (S2442) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using mTOR (S2442) pAb #BS3611.

Immunofluorescence analysis of MCF-7 cells using mTOR (S2442) antibody at dilution of 1:50.

Shionone protects cerebral ischemic injury through alleviating microglia-mediated neuroinflammation

JournalChinese Journal of Natural Medicines

ApplicationWB

IF4

SpeciesMouse

PMID40274349