产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

S6 Ribosomal Protein (RPS6) polyclonal antibody（KU）

货号: BS3610

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3610

Product Name :S6 Ribosomal Protein (RPS6) polyclonal antibody（KU）

Swiss-Prot :P62753

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:200

Background :Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :32KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RPS6;S6;RPS6

Immunogen :A synthetic peptide of human RPS6(NP_001001.2).

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of RPS6 polyclonal antibody at 1:2000 dilution Lane1:THP-1 whole cell lysate(40ug) Lane2:Jurkat whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug) Lane6:CT-26 whole cell lysate(40ug) Lane7:PC12 whole cell lysate(40ug)

Immunofluorescence analysis of PC12 cells using RPS6 antibody at dilution of 1:50.

Immunohistochemistry of paraffin-embedded human small intestine using HMGCR antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human uterine cancer using HMGCR antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Specific Disruption of Tsc1 in Ovarian Granulosa Cells Promotes Ovulation and Causes Progressive Accumulation of Corpora Lutea

JournalPLoS One

ApplicationWB

IF2.74

SpeciesMouse

PMID