产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Caspase-12 polyclonal antibody

货号: BS3542

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3542

Product Name :Caspase-12 polyclonal antibody

Swiss-Prot :Q6UXS9

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :55KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CASP12;CASP-12;CASP12P1

Immunogen :A synthetic peptide of human Caspase-12(NP_001177945.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Mouse liver, using Caspase-12 antibody at 1:840 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human tonsil using Caspase-12 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse spleen using Caspase-12 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat spleen using Caspase-12 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ER stress and ER stress-induced apoptosis are activated in gastric SMCs in diabetic rats

JournalWorld Journal Of Gastroenterology

ApplicationIHC

IF3.411

SpeciesRat

PMID25009401
Clinically used antirheumatic agent auranofin is a proteasomal deubiquitinase inhibitor and inhibits tumor growth

JournalOncotarget

ApplicationWB

IF0

SpeciesHuman

PMID24977961
The reproductive toxicology of male SD rats after PM2.5 exposure mediated by the stimulation of endoplasmic reticulum stress

JournalChemosphere

ApplicationWB

IF5.108

SpeciesRat

PMID28961540
Endoplasmic Reticulum Stress May Not Be Involved in Intestinal Epithelial Cell Apoptosis in Experimental Acute Pancreatitis

JournalDigestive Diseases And Sciences

ApplicationIHC

IF2.937

SpeciesRat

PMID25616612
Endoplasmic reticulum stress plays an important role in methotrexate-related cognitive impairment in adult rats

JournalInternational Journal of Clinical and Experimental Pathology

ApplicationWB

IF0.252

SpeciesRat

PMID31966359