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Rho H (M134) polyclonal antibody（KU）

货号: BS2660

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2660

Product Name :Rho H (M134) polyclonal antibody（KU）

Swiss-Prot :Q15669

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The Rho subfamily of small GTP-binding proteins mediates many fundamental cellular functions. The commonly studied members (Rho, Rac, and CDC42) regulate actin reorganization and affect diverse cellular responses, including adhesion, cytokinesis, and motility. RhoH, also known as TTF (Translocation Three Four), Rho-related GTP-binding protein and ras homolog gene family member H, is unlike most other small G proteins. Most small G proteins are expressed ubiquitously, however, Rho H is expressed only in hemopoietic cells and tissues. Translocations and a high frequency of Rho H mutation have been detected in primary lymphoma cells. Rho H expression has also been observed in activated neutrophils. RhoH is GTPase deficient, remaining in a GTP-bound activated state without cycling. Rho H may be involved in the functional differentiation of T cells and in cytoskeleton organization. The RhoH/TTF (ARHH) gene maps to chromosome 4p13 and encodes a 191 -amino acid polypeptide.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Rho H (M134) polyclonal antibody detects endogenous levels of Rho H protein.

BiowMW :~ 21 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Rho-related GTP-binding protein RhoH; GTP-binding protein TTF; Translocation three four protein; RHOH; ARHH; TTF

Immunogen :Synthetic peptide, corresponding to amino acids 102-152 of Human Rho H.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Rho H (M134) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(20ug) Lane2:HepG2 whole cell lysate(20ug) Lane3:Hela whole cell lysate(20ug) Lane4:C6 whole cell lysate(40ug) Lane5:CT26 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Rho H (M134) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.