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PKAα/β cat (K17) polyclonal antibody（KU）

货号: BS2648

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2648

Product Name :PKAα/β cat (K17) polyclonal antibody（KU）

Swiss-Prot :P17612/P22694

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PKAα/β cat (K17) polyclonal antibody detects endogenous levels of PKAα/β protein.

BiowMW :~ 40, 42 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACA; PKACA; PKA Ca; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACB; PKACB; PKA Cb;

Immunogen :Synthetic peptide, corresponding to the N-terminus of Human PKAα.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PKAα/β cat (K17) polyclonal antibody at 1:500 dilution Lane1:The brain tissue lysate of Rat(40ug) Lane2:The Testis tissue lysate of Mouse(40ug) Lane3:The Testis tissue lysate of Rat(40ug) Lane4:MCF-7 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PKAα/β cat (K17) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of PKAα/β cat (K17) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of PKAα/β cat (K17) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

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