产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Bcl-w (G154) polyclonal antibody（KU）

货号: BS2532

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2532

Product Name :Bcl-w (G154) polyclonal antibody（KU）

Swiss-Prot :Q92843

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRα gene on chromosome 14. Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues. An alternative splicing event in exon 4 results in two transcripts. The first, Bcl-w, encodes a protein of 193 amino acids, and the second, Bcl-w/rox, encodes a protein 333 amino acids in length. The “rox” portion of Bcl-w/rox shows a striking 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Bcl-w (G154) polyclonal antibody detects endogenous levels of Bcl-w protein.

BiowMW :~ 21 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,

Alternative Name :Bcl-2-like protein 2; Bcl2-L-2; Apoptosis regulator Bcl-W; BCL2L2; BCLW; KIAA0271

Immunogen :Synthetic peptide, corresponding to amino acids 120-165 of Human Bcl-w.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Bcl-w (G154) pAb at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:SP2/0 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Bcl-w (G154) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of Bcl-w (G154) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Silencing Prion Protein in MDA-MB-435 Breast Cancer Cells Leads to Pleiotropic Cellular Responses to Cytotoxic Stimuli

JournalPLoS One

ApplicationWB

IF2.776

SpeciesHuman

PMID23133614
Inhibition of neurotensin receptor 1 induces intrinsic apoptosis via let-7a-3p/Bcl-w axis in glioblastoma

JournalBritish Journal Of Cancer

ApplicationWB

IF5.791

SpeciesHuman

PMID28494471