产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

ZBP-89 (E84) polyclonal antibody（KU）

货号: BS2510

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2510

Product Name :ZBP-89 (E84) polyclonal antibody（KU）

Swiss-Prot :Q9UQR1

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :ZBP-89, also known as BFCOL1, BERF1 and ZNF 148, is a zinc finger transcription factor that is universally expressed. ZBP-89, a Kruppel-like repressor protein, is the silencer element binding factor for Vimentin. ZBP-89 has been shown to bind to GC-rich DNA elements in promoters for gastrin, ornithine decarboxylase and the cyclin-dependent kinase inhibitor p21 (also designated Cip1 or WAF1). ZBP-89 expression is induced by trans-retinoic acid or butyrate, which also induces terminal differentiation of colon cancer cells. ZBP-89 cooperates with histone acetyltransferase coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor whose associated gene is a target gene of p53. ZBP-89 also regulates cell proliferation, in part, through its ability to directly bind the p53 protein and retard its nuclear export. Elevated levels of ZBP-89 induce growth arrest and apoptosis in human gastrointestinal cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ZBP-89 (E84) polyclonal antibody detects endogenous levels of ZBP-89 protein.

BiowMW :~ 100-120 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Zinc finger protein 148; Transcription factor ZBP-89; Zinc finger DNA-binding protein 89; ZNF148; ZBP89

Immunogen :Synthetic peptide, corresponding to amino acids 53-98 of Human ZBP-89.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of ZBP-89 (E84) at 1:500 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:SP2/0 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:U-87MG whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of ZBP-89 (E84) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.