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ALX3 (R203) polyclonal antibody（KU）

货号: BS1898

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1898

Product Name :ALX3 (R203) polyclonal antibody（KU）

Swiss-Prot :O95076

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Aristaless-related genes are a group of paired-related homeobox genes which play a role in regulating vertebrate embryogenesis. The homeodomain transciption factor aristaless-like 3 (ALX3) is expressed in mouse embryos from 8 days of gestation, predominantly in neural crest-derived mesenchyme and in lateral plate mesoderm. Expression analysis of human and mouse tissue reveals predominant ALX3 expression in brain tissue. The Alx3 gene maps to chromosome 1p23-p13 and encodes a 343 amino acid protein. Preferential methylation of Alx3 occurs in advanced-stage neuroblastoma and may repress ALX3 expression. Treatment with the methylation inhibitor 5-aza-2’-deoxycytidine restores ALX3 expression. Alx3 (-) mice lack a phenotype distinct from wild-type mice, however Alx3/Alx4 double mutants demonstrate severe craniofacial abnormalities not present in Alx4 single mutants. Specifically, Alx3/Alx4 double mutant newborn mice have cleft nasal regions in addition to malformation of other neural crest-derived skull structures.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ALX3 (R203) polyclonal antibody detects endogenous levels of ALX3 protein.

BiowMW :~ 36 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Homeobox protein aristaless-like 3; Proline-rich transcription factor ALX3;

Immunogen :Synthetic peptide, corresponding to amino acids 171-220 of Human ALX3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ALX3 (R203) pAb at 1:1000 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:PMVEC whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of ALX3 (R203) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.