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BID (I72) polyclonal antibody（KU）

货号: BS1819

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1819

Product Name :BID (I72) polyclonal antibody（KU）

Swiss-Prot :P55957

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :BID, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :BID (I72) polyclonal antibody detects endogenous levels of BID protein.

BiowMW :~ 22 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,Death Receptor Signaling,

Alternative Name :BH3-interacting domain death agonist; p22 BID; BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID;,BH3-interacting domain death agonist p11; p11 BID; BID

Immunogen :Synthetic peptide, corresponding to amino acids 50-100 of Human BID.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of BID (I72) pAb at 1:1000 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:SK-OVCAR3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of BID (I72) pAb in paraffin-embedded human brain tissue.

Cathepsin B deficiency attenuates cardiac remodeling in response to pressure overload via TNF-α/ASK1/JNK pathway

JournalAmerican Journal Of Physiology-Heart And Circulatory Physiology

ApplicationWB

IF4.733

SpeciesMouse,Rat

PMID25713304
iTRAQ-based quantitative proteomic analysis of the anti-apoptotic effect of hyperin, which is mediated by Mcl-1 and Bid, in H2O2-injured EA.hy926 cells

JournalInternational Journal Of Molecular Medicine

ApplicationWB

IF3.098

SpeciesHuman

PMID26935776