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AKT (A444) polyclonal antibody（KU）

货号: BS1810

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1810

Product Name :AKT (A444) polyclonal antibody（KU）

Swiss-Prot :P31749/P31751/Q9Y243

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP IF

Application_all :WB 1:500 - 1:1000 IHC 1:50 - 1:100 IP 1:10 - 1:100 IF: 1:100~1:500

Background :AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AKT (A444) polyclonal antibody detects endogenous levels of total AKT protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG; PKBγ

Immunogen :Synthetic peptide, corresponding to amino acids 420-470 of Human AKT1.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of AKT (A444) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:The Lung tissue lysate of Rat(40ug) Lane3:The Lung tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of AKT (A444) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunoprecipitation of the Lung tissue lysate of Mouse using AKT (A444)pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using AKT (A444)pAb #BS1810.“↑”(supernatant); “↓”(deposition)

Immunofluorescence analysis of A2780 cells using AKT (A444) pAb at dilution of 1:200 (40x lens).

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