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Caspase 3 (D146) polyclonal antibody

货号: BS1518

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1518

Product Name :Caspase 3 (D146) polyclonal antibody

Swiss-Prot :P42574

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10).The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF).

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Caspase 3 (D146) polyclonal antibody detects endogenous levels of Caspase 3 protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,Death Receptor Signaling,

Alternative Name :Caspase-3; Caspase3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32

Immunogen :Synthetic peptide, corresponding to amino acids 120-170 of Human Caspase 3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Caspase 3 (D146) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Hela treated with H2O2 (100nmol/ml, 25min, Na3O4V) whole cell lysate (40ug) Lane3:Hela treated with H2O2 (100nmol/ml, 25min, λ-ppase) whole cell lysate (40ug)

Immunohistochemistry (IHC) analyzes of Caspase 3 (D146) pAb in paraffin-embedded human lymph node tissue.

MicroRNA-409-3p regulates cell proliferation and apoptosis by targeting PHF10 in gastric cancer

JournalCancer Letters

ApplicationWB

IF7.36

SpeciesHuman

PMID22388101
Fluoride‐induced apoptosis and expressions of caspase proteins in the kidney of carp (Cyprinus carpio)

JournalEnvironmental Toxicology

ApplicationIHC

IF2.649

SpeciesCarp

PMID24459041
Functional toll-like receptor 3 expressed by oral squamous cell carcinoma induced cell apoptosis and decreased migration

JournalOral Surgery Oral Medicine Oral Pathology Oral Radiology

ApplicationWB

IF2.589

SpeciesHuman

PMID
Decreased expression of c-kit and telomerase in a rat model of chronic endometrial ischemia

JournalMedical Science Monitor

ApplicationWB,IHC

IF1.98

SpeciesRat

PMID21455098
The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps

JournalMedical Science Monitor

ApplicationIHC

IF1.98

SpeciesHuman

PMID21804459