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CD95 (L315) polyclonal antibody（KU）

货号: BS1461

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1461

Product Name :CD95 (L315) polyclonal antibody（KU）

Swiss-Prot :P25445

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immuno- surveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FASbased mechanism involves the transducing molecule FAS (also designated APO-1) and its ligand (FAS-L). The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CD95 (L315) polyclonal antibody detects endogenous levels of CD95 protein.

BiowMW :~ 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Death Receptor Signaling,

Alternative Name :Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating; surface antigen FAS; FASLG receptor; CD95; FAS; APT1; FAS1; TNFRSF6

Immunogen :Synthetic peptide, corresponding to amino acids 280-330 of Human CD95.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of CD95 (L315) pAb at 1:500 dilution Lane1:Jurkat whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug) Lane4:SP2/0 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of CD95 (L315) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Isoflurane Damages the Developing Brain of Mice and Induces Subsequent Learning and Memory Deficits through FASL-FAS Signaling

JournalBiomed Research International

ApplicationWB

IF2.276

SpeciesMouse

PMID26609525
Saponin 6 derived from Anemone taipaiensis induces U87 human malignant glioblastoma cell apoptosis via regulation of Fas and Bcl‑2 family proteins

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesHuman

PMID27175997