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Calnexin (D577) polyclonal antibody（KU）

货号: BS1438

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1438

Product Name :Calnexin (D577) polyclonal antibody（KU）

Swiss-Prot :P27824

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100

Background :Calnexin and Calregulin (also called Calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum—Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Calnexin (D577) polyclonal antibody detects endogenous levels of Calnexin protein.

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; CANX

Immunogen :Synthetic peptide, corresponding to amino acids 540-590 of Human Calnexin.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Calnexin (D577) polyclonal antibody at 1:500 dilution Lane1:786-O whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:The kidney tissue lysate of Fetal Mouse(40ug) Lane5:The kidney tissue lysate of Fetal Rat(40ug)

Immunohistochemistry (IHC) analyzes of Calnexin (D577) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of L02 whole cell lysate using Calnexin (D577) pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5 ) .Lane 1 is 20% input. The western blot was probed using Calnexin (D577) pAb #BS1438.“↑”（supernatant）; “↓”（deposition）

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