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Caspase-8 (S347) polyclonal antibody（KU）

货号: BS1387

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1387

Product Name :Caspase-8 (S347) polyclonal antibody（KU）

Swiss-Prot :Q14790

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:2000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Caspase 8 (S347) polyclonal antibody detects endogenous levels of Caspase 8 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Necrotic Cell Death,Inhibition of Apoptosis,Toll-like Receptor signaling,Death Receptor Signaling,

Alternative Name :Caspase-8; Caspase8; Caspase 8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; Caspase-8 subunit p18; Caspase-8 subunit p10; CASP8; MCH5

Immunogen :Synthetic peptide, corresponding to Human Caspase-8.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Caspase-8 pAb at 1:2000 dilution Lane1:PC12 whole cell lysate(30ug) Lane2:BV2 whole cell lysate(30ug) Lane3:A549 whole cell lysate(30ug) Lane4:MCF-7 whole cell lysate(20ug)

Immunohistochemistry (IHC) analyzes of Caspase-8 (S347) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.

Activation of p38-MAPK by CXCL4/CXCR3 axis contributes to p53-dependent intestinal apoptosis initiated by 5-fluorouracil

JournalCancer Biology & Therapy

ApplicationWB

IF4.742

SpeciesRat

PMID24800927