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JAK2 (L1001) polyclonal antibody（KU）

货号: BS1194

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

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No. :BS1194

Product Name :JAK2 (L1001) polyclonal antibody（KU）

Swiss-Prot :O60674

Host :Rabbit

Reactivity :Human

Applications :WB IF

Application_all :WB: 1:1000~1:2000 IF: 1:50~1:200

Background :Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins.Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B).In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation.Plays a role in cell cycle by phosphorylating CDKN1B.Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :JAK2 polyclonal antibody detects endogenous levels of JAK2 protein.

BiowMW :~ 125 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2

Immunogen :Synthetic peptide, corresponding to Human JAK2.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of JAK2 polyclonal antibody at 1:1000 dilution Lane1:k562 whole cell lysate(30ug) Lane2:TF-1 whole cell lysate(30ug)

Immunofluorescence analysis of K562 cells using JAK2 (L1001) pAb at dilution of 1:50 (40x lens).

免疫沉淀（IP）

免疫组织化学（IHC）