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Claudin-4 (P192) polyclonal antibody（KU）

货号: BS1068

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1068

Product Name :Claudin-4 (P192) polyclonal antibody（KU）

Swiss-Prot :O14493

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:50~1:200

Background :The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Claudin 4 (P192) polyclonal antibody detects endogenous levels of Claudin 4 protein.

BiowMW :~ 22 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Claudin 4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein; CLDN4; CPER; CPETR1; WBSCR8

Immunogen :Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-4.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Claudin-4 (P192) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:DLD whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Claudin-4 (P192) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of HCT116 cell lysate using Claudin-4 (P192) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using Claudin-4 (P192) #BS1068. “↑”（supernatant）; “↓(deposition）

Protective role of uncoupling protein-2 against dextran sodium sulfate-induced colitis

JournalJournal Of Gastroenterology And Hepatology

ApplicationIHC

IF4.029

SpeciesMouse

PMID21871020
Claudin-4 is required for modulation of paracellular permeability by muscarinic acetylcholine receptor in epithelial cells

JournalJournal Of Cell Science

ApplicationIP,WB,IF

IF4.517

SpeciesRat

PMID25948584
Parasympathectomy increases resting secretion of the submandibular gland in minipigs in the long term

JournalJournal Of Cellular Physiology

ApplicationWB,IF

IF4.522

SpeciesPig

PMID30387129
CTRP3 promotes TNF-α-induced apoptosis and barrier dysfunction in salivary epithelial cells

JournalCellular Signalling

ApplicationWB

IF4.315

SpeciesRat

PMID33991612
Shp2 regulates PM2.5-induced airway epithelial barrier dysfunction by modulating ERK1/2 signaling pathway

JournalToxicology Letters

ApplicationWB,IF

IF3.569

SpeciesHuman,Mouse

PMID34252507