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Catenin-β (I35) polyclonal antibody（KU）

货号: BS1041

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1041

Product Name :Catenin-β (I35) polyclonal antibody（KU）

Swiss-Prot :P35222

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200 IF: 1:100~1:500

Background :β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Catenin-β (I35) polyclonal antibody detects endogenous levels of Catenin-β protein.

BiowMW :~ 92 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Hypoxia Signaling,Wntβ catenin signaling，Contribution of ECM and Cytoskeletal Factors to EMT,Contribution of Soluble Factors to EMT,hippo Signaling,Adherens Junction signaling,

Alternative Name :Catenin β; Cateninβ; Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; ORF Names: OK/SW-cl.35; PRO2286

Immunogen :Synthetic peptide, corresponding to amino acids N-terminus of Human Catenin-β.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Catenin-β (I35) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:The Uterus tissue lysate of Rat(40ug) Lane3:The Embryo tissue lysate of Mouse(40ug)

Immunofluorescence analysis of Hela cells using Catenin-β (I35) pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of Hela cell lysates using Catenin-β pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using Catenin-β pAb.