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Bad (F130) polyclonal antibody

货号: BS1027

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1027

Product Name :Bad (F130) polyclonal antibody

Swiss-Prot :Q92934

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :IHC

Application_all :IHC: 1:50~1:200

Background :Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Bad (F130) polyclonal antibody detects endogenous levels of Bad protein.

BiowMW :~ 24 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :BAD; Bcl-2-binding component 6; Bcl-XL/Bcl-2-associated death promoter; Bcl-2-like 8 protein; BAD; BBC6; BCL2L8

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human Bad.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry (IHC) analyzes of Bad (F130) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Icaritin induces lytic cytotoxicity in extranodal NK/T-cell lymphoma

JournalJournal Of Experimental & Clinical Cancer Research

ApplicationWB

IF7.068

SpeciesHuman

PMID25887673