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ATF2 (V65) polyclonal antibody

货号: BS1024

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1024

Product Name :ATF2 (V65) polyclonal antibody

Swiss-Prot :P15336

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATF2 (V65) polyclonal antibody detects endogenous levels of ATF2 protein.

BiowMW :~ 55 to 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Signaling Pathways Activating p38 MAP Kinase,

Alternative Name :Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1; ATF-2; CREB2; CREBP1

Immunogen :Synthetic peptide, corresponding to amino acids 30-80 of Human ATF2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ATF2 (V65) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse kidney tissue lysate Lane3:Rat brain tissue lysate

Immunohistochemistry (IHC) analyzes of ATF2 (V65) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Exploration of 1-(3-chloro-4-(4-oxo-4H-chromen-2-yl)phenyl)-3-phenylurea derivatives as selective dual inhibitors of Raf1 and JNK1 kinases for anti-tumor treatment

JournalBioorganic & Medicinal Chemistry

ApplicationWB

IF3.073

SpeciesHuman

PMID