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ASK1 (T77) polyclonal antibody

货号: BS1019

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1019

Product Name :ASK1 (T77) polyclonal antibody

Swiss-Prot :Q99683

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IHC

Application_all :WB:1:500~1:1000 IHC: 1:50~1:200

Background :Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ASK1 (T77) polyclonal antibody detects endogenous levels of ASK1 protein

BiowMW :~ 165 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :SAPK JNK Signaling Cascades,Signaling Pathways Activating p38 MAP Kinase

Alternative Name :Apoptosis signal regulating kinase 1; Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; ASK1; M3K5; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; Mitogen activated protein kinase kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5;

Immunogen :Synthetic peptide, corresponding to amino acids 43-90 of Human ASK1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MAP3K5 pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of ASK1 (T77) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.