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ATG5 polyclonal antibody

货号: AP6026

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP6026

Product Name :ATG5 polyclonal antibody

Swiss-Prot :Q9H1Y0

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:200

Background :Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents . Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer . The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles . This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATG5 polyclonal antibody detects endogenous levels of ATG5 protein.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling,

Alternative Name :APG5-like; Apoptosis-specific protein

Immunogen :Recombinant full length Human ATG5.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ATG 5 polyclonal antibody at 1:1000 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(30ug) Lane3:SK-OVCAR3 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:The brain tissue lysate of Mouse(40ug)

Western blot analysis of extracts from HEK293T cells transfected with control shRNA (-) (Lane 1) or ATG5 shRNA (+)(Lane 2-3). ATG5 was detected using ATG5 pAb #AP6026.The ATG5 Antibody confirms silencing of ATG5 expression.

IF image of AP6026 stained A549 cells.The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ATG5 #AP6026(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Rabbit IgG (H+L) FITC #BS10950 used at a 1/1000 dilution for 1h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).

Large-leaf yellow tea attenuates high glucose-induced vascular endothelial cell injury by up-regulating autophagy and down-regulating oxidative stress

JournalFood & Function

ApplicationWB

IF5.396

SpeciesHuman

PMID35084418
Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway

JournalFrontiers in Pharmacology

ApplicationWB

IF5.811

SpeciesRat

PMID35431972
Patient-driven discovery of CCN1 to rescue cutaneous wound healing in diabetes via the intracellular EIF3A/CCN1/ATG7 signaling by nanoparticle-enabled delivery

JournalBIOMATERIALS

ApplicationWB

IF15.304

SpeciesMouse

PMID36038422
CDK4/6 Inhibitors Impede Chemoresistance and Inhibit Tumor Growth of Small Cell Lung Cancer

JournalAdvanced Science

ApplicationWB

IF14.3

SpeciesHuman

PMID39136283
Unconjugated bilirubin promotes uric acid restoration by activating hepatic AMPK pathway

JournalFREE RADICAL BIOLOGY AND MEDICINE

ApplicationWB

IF7.1

SpeciesMouse,Human

PMID39299526