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Bcl-2 (P65) polyclonal antibody（KU）

货号: AP0368

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0368

Product Name :Bcl-2 (P65) polyclonal antibody（KU）

Swiss-Prot :Q16611

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF

Application_all :WB: 1:1000~1:2000 IF: 1:100~1:500

Background :Bak is a proapoptotic member of the Bcl-2 family. This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway. Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Bcl-2 (P65) polyclonal antibody detects endogenous levels of Bcl-2 protein.

BiowMW :~ 30 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK; BCL2L7; CDN1

Immunogen :Synthetic peptide, corresponding to Human Bcl-2.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Bcl-2 (P65) polyclonal antibody at 1:1000 dilution Lane1:The Kidney tissue lysate of Rat(30ug) Lane2:The Heart tissue lysate of Mouse(30ug)

Western blot (WB) analysis of Bcl-2 (P65) pAb at 1:1000 dilution Lane1:The Kidney tissue lysate of Rat(30ug) Lane2:A2780 whole cell lysate(30ug) Lane3:A549 whole cell lysate(30ug)

Immunofluorescence analysis of A549 cells using Bcl-2 (P65) pAb at dilution of 1:200 (40x lens).