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Caspase-3 (Phospho-S150) polyclonal antibody（KU）

货号: AP0249

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0249

Product Name :Caspase-3 (Phospho-S150) polyclonal antibody（KU）

Swiss-Prot :P42574

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:1000~1:2000

Background :Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Caspase-3 (Phospho-S150) polyclonal antibody detects endogenous levels of Caspase-3 protein only when phosphorylated at Ser150.

BiowMW :~ 33 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,Death Receptor Signaling,

Alternative Name :Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12; CASP3; CPP32

Immunogen :Synthetic phosphopeptide derived from human Caspase-3 around the phosphorylation site of Serine 150.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of Caspase-3 (Phospho-S150) polyclonal antibody at 1:1000 dilution Lane1:Jurkat whole cell lysate(30ug) Lane2:Jurkat treated with λ-phosphatase whole cell lysate(30ug)