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AKT (phospho-T308) polyclonal antibody（KU）

货号: AP0056

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :AP0056

Product Name :AKT (phospho-T308) polyclonal antibody（KU）

Swiss-Prot :P31749/P31751

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP IF

Application_all :WB: 1:2000~1:5000 IP 1:50~1:200 IF 1:50~1:200

Background :Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad, forkhead transcription factors, c-Raf, and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway. LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β. Akt may also play a role in insulin stimulation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 and p21 Waf1/Cip1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AKT (phospho-T308) polyclonal antibody detects endogenous levels of AKT protein only when phosphorylated at Thr308.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB, RAC

Immunogen :Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Threonine 308.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of AKT (phospho-T308) polyclonal antibody at 1:5000 dilution Lane1:HeLa whole cell lysate(40ug) Lane2:Hela treated with λ-phosphatase whole cell lysate(40ug) Lane3:PMVEC whole cell lysate(40ug) Lane4:BV2 whole cell lysate(40ug)

Immunofluorescence analysis of Hela cells using AKT (phospho-T308) pAb at dilution of 1:200.

Western blot (WB) analysis of AIF polyclonal antibody at 1:5000 dilution Blocking buffer: 5% BSA in TBST. Lane1:HEK293T whole cell lysate(30ug) Lane2:HepG2 whole cell lysate(30ug) Lane3:H9C2 whole cell lysate(30ug) Lane4:CT-26 whole cell lysate(30ug) Lane5:The kidney tissue lysate of Rat(30ug) Lane6:The stomach tissue lysate of Mouse(30ug)

Modified Si-Miao-San extract inhibits inflammatory response and modulates insulin sensitivity in hepatocytes through an IKKβ/IRS-1/Akt-dependent pathway

JournalJournal Of Ethnopharmacology

ApplicationWB

IF4.36

SpeciesHuman

PMID
Elevated eukaryotic elongation factor 2 expression is involved in proliferation and invasion of lung squamous cell carcinoma

JournalOncotarget

ApplicationWB

IF0

SpeciesHuman

PMID27542262
Escitalopram attenuates β-amyloid-induced tau hyperphosphorylation in primary hippocampal neurons through the 5-HT1A receptor mediated Akt/GSK-3β pathway

JournalOncotarget

ApplicationWB

IF0

SpeciesRat

PMID26950279
PKG II reverses HGF-triggered cellular activities by phosphorylating serine 985 of c-Met in gastric cancer cells

JournalOncotarget

ApplicationWB

IF0

SpeciesHuman

PMID27147579
Scutellarin, a modulator of mTOR, attenuates hepatic insulin resistance by regulating hepatocyte lipid metabolism via SREBP-1c suppression

JournalPhytotherapy Research

ApplicationWB

IF5.878

SpeciesMouse,Human

PMID31828866