Product Name :Tubulin β (4D9) monoclonal antibody
Reactivity :Human,Mouse, Rabbit, Frog, Fish, Rat(not applicable to some rat tissue)
Applications :WB IHC IF IP
Swiss-Prot :Q13509/P04350/P68371/P07437/Q13885/Q9BUF5
Application_all :WB: 1:5000~20000
IHC: 1:100~500
IF: 1:100~500
IP: 1:100~500
Background :Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da.
Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Tubulin β (4D9) mAb detects endogenous levels of β-Tubulin protein.
Figure1 :Western blot (WB) analysis of Tubulin β (4D9) mAb at 1:5000/1:10000/1:20000 dilution
Lane1:L02 whole cell lysate(20ug)
Lane2:NIH-3T3 whole cell lysate(20ug)
Lane3:PC12 whole cell lysate(20ug)
Lane4:The Kidney tissue lysate of Mouse(20ug)
Figure3 :IF image of BS1482M stained A375 cells.The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Tubulin β (4D9) mAb #BS1482M(1:500) at 10µg/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Mouse IgG (H+L) FITC#BS50950 used at a 1/400 dilution for 1h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).
Figure4 :Immunoprecipitation of MCF-7 cell lysates using Tubulin β mAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3).Lane 1 is 30% input. The western blot was probed using Tubulin β pAb.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Total tubulin β; Tubulin beta
Immunogen :Synthetic peptide, corresponding to amino acids 402-455 of Human Tubulin β.
