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CD147 polyclonal antibody（KU）

货号: NCP0177P

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :NCP0177P

Product Name :CD147 polyclonal antibody（KU）

Swiss-Prot :P35613

Host :Rabbit

Reactivity :Human

Applications :WB IP IF

Application_all :WB: 1:2000~1:5000 IP 1:50 - 1:200 IF 1:100 - 1:500

Background :Basigin (EMMPRIN, CD147) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. Basigin is a glycosylated protein with four known isoforms, of which isoform 2 is the most abundantly expressed. Multiple functions have been ascribed to Basigin; foremost among these is stimulating the secretion of extracellular matrix metalloproteinases by adjacent fibroblasts, a function which has been implicated in promoting tumor progression. Research studies have shown that Basigin is overexpressed by many tumor cells, and its expression level may correlate with tumor malignancy. A recent study identified the BASIGIN gene as a regulatory target of Slug, suggesting a role for Basigin in the process of epithelial-mesenchymal transition. Basigin has also been identified as a marker for a subset of highly suppressive regulatory T cells, and as an obligate receptor for the malarial parasite Plasmodium falciparum on human erythrocytes.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CD147 polyclonal antibody detects endogenous levels of CD147 protein.

BiowMW :~ 42 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Basigin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Hepatoma-associated antigen; HAb18G; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; CD147; BSG; UNQ6505/PRO21383

Immunogen :Recombinant protein of human CD147.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of CD147 polyclonal antibody at 1:5000 dilution Lane1:A375 cell membrane lysate(10ug) Lane2:SK-OVCAR3 cell membrane lysate(4ug)

Western blot (WB) analysis of CD147 polyclonal antibody at 1:2000 dilution Lane1:EC9706 cell membrane lysate(25ug) Lane2:A549 cell membrane lysate(25ug)

Immunofluorescence analysis of MG63 cells using CD147 pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of SK-OVCAR3 cell lysates using CD147 pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using CD147 pAb #NCP0177P.