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PKR monoclonal antibody

货号: MB66916

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66916

Product Name :PKR monoclonal antibody

Swiss-Prot :P19525

Host :Mouse

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)

Background :Protein kinase is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the 伪 subunit of the initiation factor eIF2 and also controls the activation of several transcription factors, such as NF-魏B, p53, and the Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-伪, viral infection, and serum starvation . Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation of Thr446 and Thr451 in the activation loop. Substitution of threonine for alanine at position 451 completely inactivated PKR, while a mutant with a threonine to alanine substitution at position 446 was partially active. Research studies have implicated PKR activation in the pathologies of neurodegenerative diseases, including Alzheimer's disease.

Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of PKR protein.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral Regulation of elF2

Alternative Name :PKR; PRKR; Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; PKR; Tyrosine-protein kinase EIF2AK2; p68 kinase

Immunogen :Recombinant fusion protein of human PKR. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of PKR expression in A431 (A), HepG2 (B), SHSY5Y (C) whole cell lysates.