Product Name :B-RAF monoclonal antibody
Application_all :WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)
Background :A-Raf, B-Raf, and c-Raf are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway . Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites, including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499 . p21-activated kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity. Ser338 of c-Raf corresponds to similar sites in A-Raf and B-Raf , although this site is constitutively phosphorylated in B-Raf . Inhibitory 14-3-3 binding sites on c-Raf can be phosphorylated by Akt and AMPK, respectively . While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed. Of particular interest, B-Raf contains three consensus Akt phosphorylation sites and lacks a site equivalent to Tyr341 of c-Raf . Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma . Six residues of c-Raf become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events .
Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :This antibody is purified through a protein G column.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of B-RAF protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :ESC Pluripotency and Differentiation Signaling Pathway,ErbB HER Signaling,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,Jak Stat ：IL-6 Receptor Signaling,Inhibition of Apoptosis,T cell receptor signaling
Alternative Name :BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
Immunogen :Recombinant fusion protein of human B-RAF. The exact sequence is proprietary.
Modification :Unmodification
