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Alpha-fetoglobulin monoclonal antibody

货号: MB66782

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66782

Product Name :Alpha-fetoglobulin monoclonal antibody

Swiss-Prot :P02771

Host :Mouse

Reactivity :Human

Applications :WB, IHC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Background :a-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity.

Product :Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Alpha-fetoglobulin protein.

BiowMW :~ 68 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-fetoglobulin. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Alpha-fetoglobulin expression in human fetal liver (A) whole cell lysates.

Immunohistochemical analysis of Alpha-fetoglobulin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.