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PGK1 monoclonal antibody

货号: MB66753

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66753

Product Name :PGK1 monoclonal antibody

Swiss-Prot :P00558

Host :Mouse

Reactivity :Human, Mouse

Applications :WB, IHC, FC

Application_all :WB (1/500 - 1/2000), IHC (1/50 - 1/100), FC (1/50 - 1/100)

Background :Phosphoglycerate kinases 1/2 (PGK1/2, ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) are somatically expressed, glycolytic enzymes that catalyze the transfer of a phosphoryl group from the acyl phosphate of 1,3-bisphosphoglycerate to ADP, thereby forming ATP and 3-phosphoglycerate. The human PGK gene is interrupted by 10 introns and spans 23 kilobases, and is X chromosome-linked at position Xq21.1, a region implicated in prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. In addition to influencing glycolysis, the PGK1 is secreted by tumor cells and contributes to proliferative angiogenic processes as a disulfide reductase. PGK1 mediated reduction of disulphide bonds in the serine proteinase plasmin initiates the release of the tumor blood vessel inhibitor angiostatin, an event that is critical for blood vessel formation or angiogenesis in tumor expansion and metastasis.

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of PGK1 protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of PGK1 expression in HEK293T (A), A431 (B), mouse brain (C) whole cell lysates.

Immunohistochemical analysis of PGK1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.