Product Name :EGFR monoclonal antibody
Applications :WB, IHC, FC
Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200), FC (1/10 - 1/50)
Background :The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Exons in the EGFR gene product are frequently either deleted or duplicated to produce deletion mutants (DM) or tandem duplication mutants (TDM),respectively, which are detected at various molecular weights. EGFR binds several ligands including epidermal growth factor (EGF), transforming growth factor a (TGFa), Amphiregulin and heparin binding-EGF (HB-EGF). Ligand binding promotes the internalization of EGFR via Clathrin-coated pits and its subsequent degradation in response to its intrinsic tyrosine kinase. EGFR is involved in organ morphogenesis and maintenance and repair of tissues, but upregulation of EGFR is associated with tumor progression. The oncogenic effects of EGFR include initiation of DNA synthesis, enhanced cell growth,invasion and metastasis. Abrogation of EGFR results in cell cycle arrest, apoptosis or dedifferentiation of cancer cells, suggesting that EGFR may be an effective therapeutic target.
Product :Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :This antibody is purified through a protein G column.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of EGFR protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Tumor Angiogenesis,ErbB HER Signaling,Jak Stat ：IL-6 Receptor Signaling,Phospholipase Signaling
Alternative Name :ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Immunogen :Recombinant fusion protein of human EGFR. The exact sequence is proprietary.
Modification :Unmodification
