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AIFM1 monoclonal antibody

货号: MB66693

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66693

Product Name :AIFM1 monoclonal antibody

Swiss-Prot :O95831

Host :Mouse

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background :Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis. AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli. Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation. Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis. Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain. While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis. Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of AIFM1 protein.

BiowMW :~ 67 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis, Mitochondrial Control of Apoptosis.

Alternative Name :AIF; PDCD8; Apoptosis-inducing factor 1 mitochondrial; Programmed cell death protein 8

Immunogen :Purified recombinant human AIF protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of AIFM1 expression in Hela (A), Ramos (B), HepG2 (C), MCF7 (D), Jurkat (E) whole cell lysates.