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DNA-PKcs monoclonal antibody

货号: MB66667

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66667

Product Name :DNA-PKcs monoclonal antibody

Swiss-Prot :P78527

Host :Mouse

Reactivity :Human

Applications :WB, IF/ICC, IP

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Background :DNA-dependent protein kinase (DNA-PK) is an important factor in the repair of double-stranded breaks in DNA. Cells lacking DNA-PK or in which DNA-PK is inhibited fail to show proper nonhomologous end-joining (NHEJ). DNA-PK is composed of two DNA-binding subunits (Ku70 and Ku86) and one 450 kDa catalytic subunit (DNA-PKcs). It is thought that a heterodimer of Ku70 and Ku86 binds to double-stranded DNA broken ends before DNA-PKcs binds and is activated. Activated DNA-PKcs is a serine/threonine kinase that has been shown to phosphorylate a number of proteins in vitro, including p53, transcription factors, RNA polymerase, and Ku70/Ku86. DNA-PKcs autophosphorylation at multiple sites, including Thr2609 and Ser2056, results in an inactivation of DNA-PK kinase activity and NHEJ ability. It has been demonstrated, however, that DNA-PK preferentially phosphorylates substrates before it autophosphorylates, suggesting that DNA-PK autophosphorylation may play a role in disassembly of the DNA repair machinery. Autophosphorylation at Thr2609 has also been shown to be required for DNA-PK-mediated double-strand break repair, and phosphorylated DNA-PK co-localizes with H2A.X and 53BP1 at sites of DNA damage. Phosphorylation at Ser2056 occurs in response to double-stranded DNA breaks and ATM activation.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of DNA-PKcs protein.

BiowMW :~ 450 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling, Cell Cycle G2 M DNA Damage Signaling Pathway

Alternative Name :HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460

Immunogen :Purified recombinant human DNA-PKcs protein fragments expressed in E.coli

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of DNA-PKcs expression in Hela (A), K562 (B), HL60 (C), Molt4 (D) whole cell lysates.