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EIF4E monoclonal antibody

货号: MB66600

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66600

Product Name :EIF4E monoclonal antibody

Swiss-Prot :P06730

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation. eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex. eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo. Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity. The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192. Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of EIF4E protein.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis，Translational Contral elF4 and p70 S6 Kinase

Alternative Name :EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Immunogen :Purified recombinant human eif4e protein expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of EIF4E expression in Jurkat (A), MCF7 (B), K562 (C), C6 (D), NIH3T3 (E), PC12 (F), HepG2 (G) whole cell lysates.