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DDX3X monoclonal antibody

货号: MB66592

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66592

Product Name :DDX3X monoclonal antibody

Swiss-Prot :O00571

Host :Mouse

Reactivity :Human, Mouse, Rat, Monkey

Applications :WB, IF/ICC, IP, ChIP

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50)

Background :The DEAD box family of RNA helicases is characterized in part by a common D-E-A-D amino acid motif. The family is composed of a growing number of proteins found in a wide range of organisms from bacteria to mammals. DEAD helicases have distinct biological functions in RNA metabolism and ribonucleoprotein (RNP) processing (reviewed in 1,2). DDX3 is a DEAD box family RNA helicase with diverse cellular functions. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1. DDX3 is required for hepatitis C virus (HCV) RNA replication and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC). Recent evidence suggests that DDX3 functions as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC, and aberrant subcellular localization occurs in many squamous cell carcinomas. Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression. DDX3 is phosphorylated at Thr204 and Thr323 by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation is thought to cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of DDX3X protein.

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DBX; DDX3; ATP-dependent RNA helicase DDX3X; DEAD box protein 3 X-chromosomal; DEAD box X isoform; Helicase-like protein 2; HLP2

Immunogen :Purified recombinant human DDX3 protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of DDX3X expression in Hela (A), NIH3T3 (B), C6 (C), COS7 (D), K562 (E), Jurkat (F) whole cell lysates.

免疫沉淀（IP）