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ZAP70 monoclonal antibody

货号: MB66578

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB66578

Product Name :ZAP70 monoclonal antibody

Swiss-Prot :P43403

Host :Mouse

Reactivity :Human

Applications :WB, IP

Application_all :WB (1/500 - 1/1000), IP (1/10 - 1/50)

Background :The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia. Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of ZAP70 protein.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling，PI3K AKT signaling

Alternative Name :SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Immunogen :Purified recombinant human ZAP-70 protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of ZAP70 expression in CEM (A), Jurkat (B) whole cell lysates.