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PI3K p85 beta monoclonal antibody

货号: MB66566

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB66566

Product Name :PI3K p85 beta monoclonal antibody

Swiss-Prot :O00459

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival. PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β. In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins. Protein extracts from 3T3-Src cells were profiled by PhosphoScan® to identify phosphotyrosine peptides. Tyr458 of PI3K p85 and Tyr199 of PI3K p55 were among 180 phosphopeptides and 185 phosphotyrosine sites identified.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of PI3K p85 beta protein.

BiowMW :~ 85 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :AMPK Signaling Pathway，Regulation of Actin Dynamics，Tumor Angiogenesis，Regulation of Apoptosis，ESC Pluripotency and Differentiation Signaling Pathway，ErbB HER Signaling，Jak Stat ：IL-6 Receptor Signaling，Inhibition of Apoptosis，Regulation of Microtubule Dynamics，Mitochondrial Control of Apoptosis，NF-kB Signaling，PI3K AKT signaling，Protein Kinase C Signaling，T Cell Receptor Signaling，Warburg Effect，mTOR Signaling

Alternative Name :Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta

Immunogen :Purified recombinant human PI3 Kinase p85 beta protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of PI3K p85 beta expression in C6 (A), K562 (B), MCF7 (C), U87MG (D), HCT116 (E), NIH3T3 (F), Hela (G) whole cell lysates.