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CRK monoclonal antibody

货号: MB66546

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB66546

Product Name :CRK monoclonal antibody

Swiss-Prot :P46108

Host :Mouse

Reactivity :Human

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :CrkII, a cellular homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases. The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk. The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mammalian CrkII proteins. Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase, IGF-I receptorand EGF receptor. Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecular folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of CRK protein.

BiowMW :~ 42 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics，MAPK Erk in Growth and Differentiation Pathway(，Death Receptor Signaling，SAPK JNK Signaling Cascades

Alternative Name :Adapter molecule crk; Proto-oncogene c-Crk; p38

Immunogen :Purified recombinant human CrkII protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of CRK expression in Lncap (A), A431 (B), MCF7 (C), HT1080 (D) whole cell lysates.