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Pyruvate Kinase monoclonal antibody

货号: MB66534

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66534

Product Name :Pyruvate Kinase monoclonal antibody

Swiss-Prot :P14618

Host :Mouse

Reactivity :Human, Mouse, Monkey

Applications :WB, IF/ICC, IP

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Background :Pyruvate kinase is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2) is an alternatively spliced variant of M1 that is expressed during embryonic development. Research studies found that cancer cells exclusively express PKM2. PKM2 is shown to be essential for aerobic glycolysis in tumors, known as the Warburg effect. When cancer cells switch from the M2 isoform to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased. These cells also show decreased tumorigenicity in mouse xenografts. Recent studies showed that PKM2 is not essential for all tumor cells. In the tumor model studied, PKM2 was found to be active in the non-proliferative tumor cell population and inactive in the proliferative tumor cell population.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of Pyruvate Kinase protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Warburg Effect

Alternative Name :OIP3; PK2; PK3; PKM2; Pyruvate kinase PKM; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58

Immunogen :Purified recombinant human PKM2 protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Pyruvate Kinase expression in HCT116 (A), COS7 (B), K562 (C), A549 (D), Hela (E) whole cell lysates.