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CDK5 monoclonal antibody

货号: MB66517

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66517

Product Name :CDK5 monoclonal antibody

Swiss-Prot :Q00535

Host :Mouse

Reactivity :Human, Mouse, Rat, Monkey

Applications :WB, IF/ICC

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Background :Cyclin-dependent kinases (CDKs) are serine/threonine kinases that are activated by cyclins and govern eukaryotic cell cycle progression. While CDK5 shares high sequence homology with its family members, it is thought mainly to function in postmitotic neurons to regulate the cytoarchitecture of these cells. Analogous to cyclins, the regulatory subunits p35 and p39 associate with and activate CDK5 despite the lack of sequence homology. CDK5 is ubiquitously expressed, with high levels of kinase activity detected primarily in the nervous system due to the narrow expression pattern of p35 and p39 in post-mitotic neurons. A large number of CDK5 substrates have been identified although no substrates have been specifically attributed to p35 or p39. Substrates of CDK5 include p35, PAK1, Src, β-catenin, tau, neurofilament-H, neurofilament-M, synapsin-1, APP, DARPP32, PP1-inhibitor, and Rb. p35 is rapidly degraded (T1/2 <20 min) by the ubiquitin-proteasome pathway. However, p35 stability increases as CDK5 kinase activity decreases, likely as a result of decreased phosphorylation of p35 at Thr138 by CDK5 . Proteolytic cleavage of p35 by calpain produces p25 upon neurotoxic insult, resulting in prolonged activation of CDK5 by p25. Research studies have shown accumulation of p25 in neurodegenerative diseases, such as Alzheimer's disease and amyotrophic lateral sclerosis (ALS).

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of CDK5 protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer\'s Disease

Alternative Name :CDKN5; Cyclin-dependent kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit

Immunogen :Purified recombinant human CDK5(N-terminus) protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of CDK5 expression in NIH3T3 (A), Hela (B), PC12 (C), COS7 (D), Jurkat (E), K562 (F) whole cell lysates.