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RAD51A monoclonal antibody

货号: MB66512

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66512

Product Name :RAD51A monoclonal antibody

Swiss-Prot :Q06609

Host :Mouse

Reactivity :Human, Mouse, Rat, Monkey

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination (HR). Research studies have shown that defects in both pathways are associated with human disease, including cancer. DSBs that arise during S or G2 phase are repaired via homologous recombination (HR), using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homologue of E. coli RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 . BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D) and they all appear to be required for efficient HR. Researchers have found that mutations in the Rad51 gene may be related to breast cancer risk. Some studies have implicated Rad51 as a potential marker for pancreatic cancer.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of RAD51A protein.

BiowMW :~ 37 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune checkpoint signaling in the Tumor Microenvironment

Alternative Name :RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A

Immunogen :Recombinant protein of human Rad51.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of RAD51A expression in CHOK1 (A), COS7 (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates.