产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

ACLY monoclonal antibody

货号: MB66502

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB66502

Product Name :ACLY monoclonal antibody

Swiss-Prot :P53396

Host :Mouse

Reactivity :Human, Mouse, Monkey

Applications :WB, IF/ICC, FC

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), FC (1/50 - 1/100)

Background :ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis . Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase . It is phosphorylated by GSK-3 on Thr446 and Ser450 . Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt . Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme .

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of ACLY protein.

BiowMW :~ 125 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ATP-citrate synthase; ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme

Immunogen :Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of ACLY expression in NIH3T3 (A), K562 (B), COS7 (C), Hela (D) whole cell lysates.

Immunofluorescent analysis of ACLY staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.